{"text_format":"3","workbook":"xls","n_sheets":4,"sheets":["Sheet1","填写示例","纯化说明","修饰说明"],"not_rendered":{"images":1,"external_links":1,"defined_names":1},"read":"whole","date_system":"1900","formulas":"cached_only"}

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[" 引物合成订购单"]
[]
[]
["订购日期",null,null,"乙方填写",{"$nulls":3},"常用兼并碱基代码",{"$nulls":3},"订单要求：\n1.此表格仅针对E-mail订单。\n2.为方便订单的处理，请您详细填写表格中的信息。                                                                     "]
["客户姓名",null,null,"订单编号"]
["负责人姓名",null,null,"总碱基数"]
["客户单位",null,null,"电话：",{"$nulls":3},"M:(A/C)",null,"V:(A/C/G)"]
["客户电话",{"$nulls":6},"R:(A/G)",null,"H:(A/C/T)"]
["E-mail",null,null,"邮箱：lishanbio666@163.com ",{"$nulls":3},"W:(A/T)",null,"D:(A/G/T)"]
["客户地址",null,null,"地址：济南市历下区经十路明湖国际细胞医学产业园D栋8F",{"$nulls":3},"S:(C/G)",null,"B:(C/G/T)"]
["发票抬头",{"$nulls":6},"Y:(C/T)",null,"N:(A/G/C/T)"]
["温馨提示：","为防止漏单而耽搁您的实验，若您在一天内没有收到本公司的确认回复邮件，请及时电话查询。   ",{"$nulls":5},"K:(G/T)"]
["序号","Primer名称","序列(5'to3')","碱基数","合成总量（OD）","分装管数","纯化方式\n（可选脱盐/PAGE）","引物用途","5'修饰","3'修饰","中间修饰","备注"]
[1,null,null,0,2,1,"DSL(脱盐)","普通PCR克隆"]

{"sheet":"填写示例","index":1,"n_rows":29,"n_cols":12,"rows_kept":29,"flags":["formulas_cached_only"]}
["常规引物下单填写示例："]
["序号","Primer名称","序列(5'to3')","碱基数","合成总量（OD）","分装管数","纯化方式","引物用途","5'修饰","3'修饰","中间修饰","备注"]
[1,"P1","GCATGCATGCATGCATGCAT",20,2,1,"DSL"]
[2,"P2","GCATGCATGCATGCATGCAT",20,4,2,"PAGE"]
[3,"P3","GCATGCATGCATGCATGCAT",20,10,2,"HPLC"]
[]
["5'端修饰填写示例："]
["序号","Primer名称","序列(5'to3')","碱基数","合成总量（OD）","分装管数","纯化方式","引物用途","5'修饰","3'修饰","中间修饰","备注"]
[1,"P1","GCATGCATGCATGCATGCAT",20,2,1,"HPLC",null,"5' PHO"]
[2,"P2","GCATGCATGCATGCATGCAT",20,4,1,"HPLC",null,"5' FAM"]
[3,"P3","GCATGCATGCATGCATGCAT",20,10,2,"HPLC",null,"5' Biotin"]
[]
["3'修饰填写示例"]
["序号","Primer名称","序列(5'to3')","碱基数","合成总量（OD）","分装管数","纯化方式","引物用途","5'修饰","3'修饰","中间修饰","备注"]
[1,"P1","GCATGCATGCATGCATGCAT",20,2,1,"HPLC",null,null,"3`BHQ1"]
[2,"P2","GCATGCATGCATGCATGCAT",20,4,1,"HPLC",null,null,"3’HEX"]
[3,"P3","GCATGCATGCATGCATGCAT",20,10,2,"HPLC",null,null,"3' DIG"]
[]
["5'端和3’端双标记修饰填写示例："]
["序号","Primer名称","序列(5'to3')","碱基数","合成总量（OD）","分装管数","纯化方式","引物用途","5'修饰","3'修饰","中间修饰","备注"]
[1,"P1","GCATGCATGCATGCATGCAT",20,2,1,"HPLC",null,"5' FAM","3' TAMRA"]
[2,"P2","GCATGCATGCATGCATGCAT",20,4,1,"HPLC",null,"5' HEX","3`BHQ1"]
[3,"P3","GCATGCATGCATGCATGCAT",20,10,2,"HPLC",null,"5' TET","3' BHQ2"]
[]
["中间修饰填写示例："]
["序号","Primer名称","序列(5'to3')","碱基数","合成总量（OD）","分装管数","纯化方式","引物用途","5'修饰","3'修饰","中间修饰","备注"]
[1,"P1","GCATGCATGCATG(*)CATGCAT",23,2,1,"HPLC",{"$nulls":3},"Phosphorothioates(13)"]
[2,"P2","GCATGCATGCATGCA(FAM)TGCAT",25,4,1,"HPLC",{"$nulls":3},"FAM(15)"]
[3,"P3","GCATGC(Biotin)ATGCATGCATGCAT",28,10,2,"HPLC",{"$nulls":3}," Biotin(6)"]

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["应用","纯化方法"]
[null,"DSL","PAGE","HPLC"]
["一般PCR扩增","√","√","√"]
["测序",null,"√","√"]
["全基因合成","√","√","√"]
["亚克隆，点突变",null,"√","√"]
["反义核酸",null,null,"√"]
["修饰引物",null,null,"√"]
["\"√\"表示推荐的纯化方式"]
[]
[]
[]
["纯化","适用长度","介绍",null,"优点"]
["DSL纯化","5-90net","又称为简易反向柱，可以被有机溶液洗脱，但不会被水洗脱，能有效的去除盐分",null,"速度快，价格优，质量高","别称+G14"]
["PAGE纯化","5-60net","针对60net以下的普通引物，推出的PAGE级别的纯化方式",null,"纯化速度快，质量更稳定"]
["HPLC纯化","5-60net","采用高效液相色谱的原理，能达到很高的纯度和灵敏度，特别适用于短链引物（<40net）引物和修饰引物纯化",null,"引物纯度大于95%，对纯化短链引物（<40net）特别有效"]

{"sheet":"修饰说明","index":3,"n_rows":141,"n_cols":10,"rows_kept":141,"merged":[[0,1,0,10],[1,0,1,4],[1,6,1,10],[2,1,3,1],[2,2,3,2],[2,3,3,3],[2,6,63,6],[2,7,3,7],[2,8,3,8],[2,9,3,9],[2,10,63,10],[4,1,13,1],[4,7,8,7],[9,7,13,7],[14,7,23,7],[15,1,16,1],[17,1,19,1],[20,1,21,1],[22,1,23,1],[24,1,25,1],[24,7,25,7],[26,1,82,1],[26,7,31,7],[32,7,39,7],[40,7,48,7],[49,7,62,7],[61,0,141,0],[61,4,141,4],[63,7,63,9],[83,1,93,1],[94,1,110,1],[111,1,140,1],[141,1,141,3]],"flags":["formulas_cached_only"]}
[null,"修饰类型"]
["单标与普通修饰",{"$nulls":5},"双标修饰引物"]
[null,"分   类","修饰标记","纯化方式",{"$nulls":3},"分   类","修饰标记","纯化方式"]
[]
[null,"稀有碱基","dI","PAGE",{"$nulls":3},"TAMRA","5' FAM-3' TAMRA","HPLC"]
[null,null,"dU","PAGE",{"$nulls":4},"5' HEX-3' TAMRA","HPLC"]
[null,null,"m5C","PAGE",{"$nulls":4},"5' TET-3' TAMRA","HPLC"]
[null,null,"m6A","PAGE",{"$nulls":4},"5' JOE-3' TAMRA","HPLC"]
[null,null,"2'-O-Methyl 碱基","PAGE",{"$nulls":4},"5＇VIC-3＇TAMRA","HPLC"]
[null,null,"RNA 碱基","PAGE",{"$nulls":3},"BHQ1","5' FAM-3' BHQ1","HPLC"]
[null,null,"3'-纤维素 dT","PAGE",{"$nulls":4},"5' VIC-3' BHQ1","HPLC"]
[null,null,"DC-Br","PAGE",{"$nulls":4},"5' HEX-3' BHQ1","HPLC"]
[null,null,"LNA-X","PAGE",{"$nulls":4},"5' JOE-3' BHQ1","HPLC"]
[null,null,"5-Methyl dC","PAGE",{"$nulls":4},"5' TET-3' BHQ-1","HPLC"]
[null,"硫代修饰(Phosphorothioates)","S","PAGE",{"$nulls":3},"BHQ2","5' Cy5-3' BHQ2","HPLC"]
[null,"磷酸基团 (Phosphorylation)","5'PHO","PAGE",{"$nulls":4},"5' Cy3-3' BHQ2","HPLC"]
[null,null,"3' PHO","PAGE",{"$nulls":4},"5' ROX-3' BHQ2","HPLC"]
[null,"NH2修饰(Amino Linkers)","5'NH2 C6","HPLC",{"$nulls":4},"5' TET-3' BHQ2","HPLC"]
[null,null,"3' NH2 C7","HPLC",{"$nulls":4},"5'JOE-3'BHQ2","HPLC"]
[null,null,"5' NH2 C12","HPLC",{"$nulls":4},"5'HEX-3'BHQ2","HPLC"]
[null,"SH修饰(Thiol)","3'SH C3","HPLC",{"$nulls":4},"5'TAMRA-3'BHQ2","HPLC"]
[null,null,"5' SH C6","HPLC",{"$nulls":4},"5'Texas Red-3'BHQ2","HPLC"]
[null,"生物素(Biotin)","5'Biotin","HPLC",{"$nulls":4},"5'Quasar 670-3'BHQ2","HPLC"]
[null,null,"3'Biotin TEG","HPLC",{"$nulls":4},"5'-FAM and 3'-BHQ2","HPLC"]
[null,"地高辛(Digoxigenin)","5'DIG","HPLC",{"$nulls":3},"BHQ3","5'Quasar 670-3'BHQ3","HPLC"]
[null,null,"3' DIG","HPLC",{"$nulls":4},"5'CY5-3'BHQ3","HPLC"]
[null,"荧光基团(Fluorescence)","5'Cy3","HPLC",{"$nulls":3},"ECLIPS","5' FAM-3' ECLIPS","HPLC"]
[null,null,"3'Cy3","HPLC",{"$nulls":4},"5' HEX-3'  ECLIPS","HPLC"]
[null,null,"5'Cy5","HPLC",{"$nulls":4},"5'TAMRA-3'Eclipse","HPLC"]
[null,null,"3'Cy5","HPLC",{"$nulls":4},"5'ROX-3'Eclipse","HPLC"]
[null,null,"5'FAM","HPLC",{"$nulls":4},"5＇TET-3＇Eclipse","HPLC"]
[null,null,"3'FAM","HPLC",{"$nulls":4},"5' JOE-3' Eclipse","HPLC"]
[null,null,"5'HEX","HPLC",{"$nulls":3},"MGB","5' FAM-3'MGB","HPLC"]
[null,null,"5'TET","HPLC",{"$nulls":4},"5' HEX-3'MGB","HPLC"]
[null,null,"5'-FITC","HPLC",{"$nulls":4},"5'TET-3'MGB","HPLC"]
[null,null,"3'-FITC","HPLC",{"$nulls":4},"5'JOE-3'MGB","HPLC"]
[null,null,"5'6-JOE","HPLC",{"$nulls":4},"5'ROX-3'MGB","HPLC"]
[null,null,"3'6-JOE","HPLC",{"$nulls":4},"5'Texas Red-3'MGB","HPLC"]
[null,null,"5'ROX","HPLC",{"$nulls":4},"5'Quasar 670-3'MGB","HPLC"]
[null,null,"3'ROX","HPLC",{"$nulls":4},"5' VIC-3'MGB","HPLC"]
[null,null,"5'TAMRA","HPLC",{"$nulls":3},"Dabcyl","5' FAM-3' DABCYL","HPLC"]
[null,null,"3'TAMRA","HPLC",{"$nulls":4},"5' HEX-3' DABCYL","HPLC"]
[null,null,"3'-HEX","HPLC",{"$nulls":4},"5' TET-3' DABCYL","HPLC"]
[null,null,"3'-TET","HPLC",{"$nulls":4},"5' JOE-3' DABCYL","HPLC"]
[null,null,"5'Helix Flour555","HPLC",{"$nulls":4},"5' TAMRA-3' DABCYL","HPLC"]
[null,null,"3'Helix Flour555","HPLC",{"$nulls":4},"5'Texas Red-3'Dabcyl","HPLC"]
[null,null,"5'Helix Flour575","HPLC",{"$nulls":4},"5'ROX-3'Dabcyl","HPLC"]
[null,null,"3'Helix Flour575","HPLC",{"$nulls":4},"5'CY3-3'DABCYL","HPLC"]
[null,null,"5'Texas Red","HPLC",{"$nulls":4},"5' Cy5-3' DABCYL","HPLC"]
[null,null,"3'Texas Red","HPLC",{"$nulls":3},"其他双标","5'Phos and 3'FAM","HPLC"]
[null,null,"5'Quasar 570","HPLC",{"$nulls":4},"5'TAMRA and 3'Phos","HPLC"]
[null,null,"3'Quasar 570","HPLC",{"$nulls":4},"5＇C6-Biotin,3＇Biotin","HPLC"]
[null,null,"5'Quasar 670","HPLC",{"$nulls":4},"5＇C6-Biotin,3＇Cy5","HPLC"]
[null,null,"3'Quasar 670","HPLC",{"$nulls":4},"5＇C6-NH2,3＇C3-Fam","HPLC"]
[null,null,"5'Cy3.5","HPLC",{"$nulls":4},"5＇C6-NH2,3＇Cy3","HPLC"]
[null,null,"3'Cy3.5","HPLC",{"$nulls":4},"5＇Cy5,SS,","HPLC"]
[null,null,"5'Cy5.5NS","HPLC",{"$nulls":4},"5＇Cy5,3＇C7-NH2","HPLC"]
[null,null,"3'Cy5.5NS","HPLC",{"$nulls":4},"5＇Cy5,3＇SH","HPLC"]
[null,null,"5'Cy7NS","HPLC",{"$nulls":4},"5＇Dabcyl,3＇C3-Fam","HPLC"]
[null,null,"3'Cy7NS","HPLC",{"$nulls":4},"5＇DlG,3＇Digoxin","HPLC"]
[null,null,"5'&3' CAL Fluor Red 590","HPLC",{"$nulls":4},"5＇SH,3＇Cy3","HPLC"]
[null,null,"5'&3'CAL Fluor Red 610","HPLC",{"$nulls":4},"5＇Fam,3＇C3-Fam","HPLC"]
[null,null,"5'&3'DABCYL","HPLC",{"$nulls":4},"5＇TAMRA，3＇C3-Fam","HPLC"]
[null,null,"5'&3'AMCA","HPLC"]
[null,null,"5'&3'DEAC","HPLC"]
[null,null,"5'&3'MCA","HPLC"]
[null,null,"5'&3'LRB Red","HPLC"]
[null,null,"5'&3'CR6G","HPLC"]
[null,null,"5'&3' Bodi Fluor R6G","HPLC"]
[null,null,"5'&3'NBD-X","HPLC"]
[null,null,"5'&3'California Red","HPLC"]
[null,null,"5'&3'iFlour系列","HPLC"]
[null,null,"5'-VIC","HPLC"]
[null,null,"3'-LC RED-610","HPLC"]
[null,null,"3'-LC RED-640","HPLC"]
[null,null,"3'-LC RED-705","HPLC"]
[null,null,"3'-Bodipy 493/503","HPLC"]
[null,null,"3'-Bodipy 564/570","HPLC"]
[null,null,"3'-Bodipy 581/591","HPLC"]
[null,null,"3'-Bodipy 630/650","HPLC"]
[null,null,"3'-Bodipy 650/665","HPLC"]
[null,null,"3'-BODIPY R6G","HPLC"]
[null,null,"5'-SIMA","HPLC"]
[null,"淬灭基团(Quenchers)","3'DABCYL","HPLC"]
[null,null,"3'TAMRA","HPLC"]
[null,null,"3'BHQ 1","HPLC"]
[null,null,"3'BHQ 2","HPLC"]
[null,null,"3'BHQ3","HPLC"]
[null,null,"3'-MGB","HPLC"]
[null,null,"3'BBQ-650","HPLC"]
[null,null,"3'TQ1-TQ6系列","HPLC"]
[null,null,"3'-QSY 7 carboxylic acid","HPLC"]
[null,null,"3'TQ7","HPLC"]
[null,null,"3'eclipse","HPLC"]
[null,"其他修饰","Spacer9(空间子)","HPLC"]
[null,null,"Azide(叠氮化)","HPLC"]
[null,null,"DBCO","HPLC"]
[null,null,"Spacer(C6)","HPLC"]
[null,null,"Spacer9","HPLC"]
[null,null,"Spacer(C12)","HPLC"]
[null,null,"Spacer C3","HPLC"]
[null,null,"Spacer C18","HPLC"]
[null,null,"dSpacer","HPLC"]
[null,null,"Inverted Base","HPLC"]
[null,null,"5'-Carboxy C10(羧基)","HPLC"]
[null,null,"5'-D4PA","HPLC"]
[null,null,"5'-Acridine","HPLC"]
[null,null,"3'ddC","HPLC"]
[null,null,"Alexa Flour 647","HPLC"]
[null,null,"Yakima Yellow Epoch","HPLC"]
[null,null,"Pyrene","HPLC"]
[null,"中间修饰","Int NH2 dT","HPLC"]
[null,null,"Int Biotin dT","HPLC"]
[null,null,"Int Cy3 dT","HPLC"]
[null,null,"Int Cy5 dT","HPLC"]
[null,null,"Int TAMRA dT","HPLC"]
[null,null,"Int ROX dT","HPLC"]
[null,null,"Int FAM dT","HPLC"]
[null,null,"Int HEX dT","HPLC"]
[null,null,"Int Dig dT","HPLC"]
[null,null,"Int CHCH dT","HPLC"]
[null,null,"Int DBCO dT","HPLC"]
[null,null,"Int Azide dT","HPLC"]
[null,null,"Int TAMRA dT","HPLC"]
[null,null,"Int ATTO Flour dT","HPLC"]
[null,null,"Int BHQ1 dT","HPLC"]
[null,null,"Int BHQ2 dT","HPLC"]
[null,null,"Int HS-SH C6","HPLC"]
[null,null,"Int Alexa Flour dT","HPLC"]
[null,null,"Int Azobenzene","HPLC"]
[null,null,"Int Ferrocene dT","HPLC"]
[null,null,"dT- LC RED-610","HPLC"]
[null,null,"dT- LC RED-640","HPLC"]
[null,null,"dT- LC RED-705","HPLC"]
[null,null,"dC-Fluorescein","HPLC"]
[null,null,"dC-Tamra","HPLC"]
[null,null,"dC-Amino","HPLC"]
[null,null,"dC-Rox","HPLC"]
[null,null,"dC-Digoxin","HPLC"]
[null,null,"dC-FITC","HPLC"]
[null,null,"dC-Texas Red","HPLC"]